人口腔鳞癌细胞

人口腔

上皮样

贴壁生长

The HSC-3 cell line was established from tumors of metastatic lymph nodes originated in tongue squamous cell carcinomas of a 63-year old male patient. Epithelial cells that migrated and proliferated were collected and subcultured from fresh explanted tumor tissues (2).

MEM+ 10%（Gibco）胎牛血清  温度：37℃ 气相：95%空气，5%二氧化碳

两天换液一次

1：2传代，消化2-3分钟

冻存液：90%FBS，DMSO 10%