小鼠软骨前体细胞

小鼠软骨

上皮样

贴壁生长

Mouse 129 teratocarcinoma AT805 derived.The chondrogenic cell line ATDC5 has been derived from teratocarcinoma AT805. Cells showed a sequential transition of phenotype in vitro. They encompass stages from mesenchymal condensation to calcification. Bone morphogenetic protein-2 (BMP-2) stimulated the sequential progression of early- and late-phase differentiation. Activation of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor resulted in inhibition of this differentiation capacity.

DMEM(高糖）+10%FBS 温度：37℃  气相：95%空气，5%二氧化碳

两天换液一次

1：2传代，消化2-3分钟

冻存液：90%FBS，DMSO 10%