产品名称
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皮肤恶性黑色素瘤细胞SK-MEL-5
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商品货号 | WN-49793 |
种属 | 人 |
别称 | SK-Mel-5; SK MEL 5; SK.MEL.5; SK-MEL5; SKMel-5; SKMEL-5; SKMEL5; SKMel5; SKmel5; AA-Mel |
组织来源 | 皮肤 |
疾病 | 皮肤黑色素瘤 |
传代比例/细胞消化 | 1:2传代,消化2-3分钟 |
简介 | SK-MEL-5 is a melanoma cell line isolated from skin tissue obtained from a 24-year-old, White, female malignant melanoma patient. This cell line is a suitable transfection host and also serves as a source of target cells for the detection of melanoma-specific antibody in patients with the disease. This is one of a very extensive series of melanoma lines isolated by T. Takahashi and associates. |
形态 | 星状细胞样 |
生长特征 | 贴壁生长 |
倍增时间 | 每周 2 至 3 次 |
抗原表达 | Blood Type O; Rh+; HLA A2, A11, B40, Bw16 |
致瘤性 | Yes, in nude mice; forms malignant melanoma |
STR | Amelogenin: X CSF1PO: 10,13 D13S317: 10,12 D16S539: 10,12 D5S818: 11,13 D7S820: 9,12
THO1: 6,9 TPOX: 11 vWA: 14,18 |
培养条件 | MEM培养基;10%胎牛血清;1%双抗气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 |
冻存条件 | 冻存液:90%FBS,DMSO 10% |
保藏机构 | ATCC;?HTB-70 |
产品使用 | 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。 |